Coding
pdc

Part:BBa_K173016:Design

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 480
    Illegal AgeI site found at 1686
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence encoded by this gene has been found in NCBI Entrez Protein retrieval system. The organism was Zymomonas mobilis ZM4. The gene sequence has been optimized for the codon usage of Escherichia coli and a second stop codon (TAA) has been added to the gene.

Source

Mr Gene synthesis service.

References

Zhanchao Wang, Ming Chen, Yuquan Xu, Shuying Li, Wei Lu, Shuzhen Ping, Wei Zhang and Min Lin, "An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose". Biotechnology Letters, Volume 30, Number 4 / April, 2008.